Sustained Release of Growth Factors from Photoactivated PRP Plasma
Introduction
Transcription of the article from the European Journal of Pharmaceutics and Biopharmaceutics, published on November 30, 2019
The purpose of this study is to specify the capacity of the polychromatic light source (PAC), providing effective wavelengths in the range of 600-1200 nm (near-infrared region, NIR), to activate human platelets in platelet-rich plasma (PRP) and achieve sustained and controlled release of growth factors from photoactivated platelets.
Methods
The PRP was isolated from human blood and treated with PAC at different time intervals for 1, 5, and 10 minutes at a distance of 10 cm from the platelets. ATP secretion, followed by calcium release from the platelets, significantly increased after a light application.
Results
Photostimulation of platelets triggered lamellipodia extension, the formation of numerous filopodia, and platelet aggregation as indicators of activation.
The expression of P-selectin was significantly increased after PAC application.
Conclusions
The PRP was successfully activated with PAC for 10 minutes and achieved sustained activation-dependent growth factor release for 28 days.
We have demonstrated that PAC, which has a high PRP activation potential, enables sustained release of growth factor from PRP with periodic use for therapeutic PRP applications.
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